4NL8
PriA Helicase Bound to SSB C-terminal Tail Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.610, 153.248, 192.373 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 4.080 |
R-factor | 0.24341 |
Rwork | 0.240 |
R-free | 0.29848 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.317 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (MR) |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.170 |
High resolution limit [Å] | 4.080 | 4.080 |
Number of reflections | 21752 | |
<I/σ(I)> | 10.91 | 1.89 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M ammonium acetate, 0.015 M magnesium acetate tetrahydrate, 0.05 M sodium cacodylate trihydrate pH 6.5, and 10% v/v 2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |