4NHO

Structure of the spliceosomal DEAD-box protein Prp28

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.001212
Spacegroup name	C 2 2 21
Unit cell lengths	125.430, 136.770, 73.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.825 - 2.000
R-factor	0.1943
Rwork	0.193
R-free	0.21760
Structure solution method	SAD
Starting model (for MR)	2db3
RMSD bond length	0.012
RMSD bond angle	1.276
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.12)
Phasing software	SOLOMON
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.970	39.970	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge	0.064	0.026	0.584
Number of reflections	42906
<I/σ(I)>	12.1	30.2	2.3
Completeness [%]	100.0	99.1	100
Redundancy	4.9	4.6	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	2.2 M ammonium sulfate, 20 % (v/v) glycerol, 0.1 M CAPS, mixing 1 ul of protein with 1 ul of reservoir solution, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	9	298	2.2 M ammonium sulfate, 20 % (v/v) glycerol, 0.1 M CAPS, mixing 1 ul of protein with 1 ul of reservoir solution, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K