4NHK
Crystal structure of Tpa1p from Saccharomyces cerevisiae, termination and polyadenylation protein 1, in complex with pyridine-2,4-dicarboxylic acid (2,4-PDCA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-26 |
| Detector | PILATUS 6M-F |
| Wavelength(s) | 0.83440 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.044, 67.716, 70.871 |
| Unit cell angles | 90.00, 104.93, 90.00 |
Refinement procedure
| Resolution | 48.630 - 1.900 |
| Rwork | 0.155 |
| R-free | 0.17580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kt7 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.3) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.066 | 0.040 | 0.729 |
| Number of reflections | 59886 | ||
| <I/σ(I)> | 8.9 | 35.7 | 2.5 |
| Completeness [%] | 99.0 | 99.4 | 98.2 |
| Redundancy | 6.6 | 6.5 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.8 MnCl2, 1.1 mM 2,4-PDCA, 0.1 M SPG buffer, 25% PEG 1500, vapor diffusion sitting drop, temperature 293K, pH 8.0, VAPOR DIFFUSION, SITTING DROP |
