4NGF

Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 17-mer siRNA having 5'-p and UU-3' ends (3.1 Angstrom resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.07500
Spacegroup name	C 2 2 21
Unit cell lengths	99.245, 104.191, 368.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.478 - 3.101
R-factor	0.2255
Rwork	0.223
R-free	0.27030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ngd
RMSD bond length	0.006
RMSD bond angle	1.165
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.210
High resolution limit [Å]	3.100	6.670	3.100
Rmerge	0.120	0.056	0.650
Number of reflections	32098
<I/σ(I)>	7.4
Completeness [%]	91.6	99.7	87.8
Redundancy	13.1	13.5	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.08 M HEPES sodium, pH 7.5, 8% 2-propanol, 16% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K