4NGB

Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 12-mer siRNA having UU-3' ends (2.25 Angstrom resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-11
Detector	ADSC QUANTUM 315
Wavelength(s)	1.08090
Spacegroup name	C 1 2 1
Unit cell lengths	109.455, 84.680, 51.634
Unit cell angles	90.00, 113.30, 90.00

Refinement procedure

Resolution	31.995 - 2.250
R-factor	0.1844
Rwork	0.182
R-free	0.21970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ngd
RMSD bond length	0.009
RMSD bond angle	1.131
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.330
High resolution limit [Å]	2.250	4.850	2.250
Rmerge	0.099	0.060	0.404
Number of reflections	20573
<I/σ(I)>	12.3
Completeness [%]	99.8	97.9	100
Redundancy	6.9	6.7	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	293	0.8 M sodium/potassium tartrate, 0.1 M sodium citrate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K