4NEO
Structure of BlmI, a type-II acyl-carrier-protein from Streptomyces verticillus involved in bleomycin biosynthesis
Replaces: 4I4DExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-27 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97931 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 73.441, 73.441, 43.331 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.1808 |
Rwork | 0.180 |
R-free | 0.20070 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.777 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.070 | 0.429 |
Number of reflections | 8055 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 7.1 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 289 | protein was reductively methylated, & crystallized in 0.1M CHES:NaOH pH 9.5, 30% PEG 400 , VAPOR DIFFUSION, SITTING DROP, temperature 289K |