Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

4NCS

Human sialidase 2 in complex with 2,3-difluorosialic acid (covalent intermediate)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron sitePhoton Factory
BeamlineAR-NW12A
Temperature [K]100
Detector technologyCCD
Collection date2010-11-09
DetectorADSC QUANTUM 210r
Wavelength(s)1
Spacegroup nameC 2 2 21
Unit cell lengths88.144, 88.256, 92.684
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution31.200 - 2.201
R-factor0.16385
Rwork0.161
R-free0.22451
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1vcu
RMSD bond length0.016
RMSD bond angle1.918
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareMOLREP
Refinement softwareREFMAC (5.7.0032)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]31.2002.250
High resolution limit [Å]2.2002.200
Number of reflections9808
Completeness [%]99.899.56
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8289Sodium/potassium phosphate buffer, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 289K

239803

PDB entries from 2025-08-06

PDB statisticsPDBj update infoContact PDBjnumon