4NCF

Crystal structure of eukaryotic translation initiation factor eIF5B (399-852) from Saccharomyces cerevisiae in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.826580
Spacegroup name	P 21 21 21
Unit cell lengths	73.560, 119.460, 120.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.664 - 3.015
R-factor	0.254
Rwork	0.253
R-free	0.28080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n3s
RMSD bond length	0.003
RMSD bond angle	0.810
Data scaling software	XSCALE
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.664		3.120
High resolution limit [Å]	3.020	50.000	3.020
Rmerge	0.053		0.606
Number of reflections	21521
<I/σ(I)>	23.32		3.52
Completeness [%]	99.2		99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	8 % PEG 8000 0.37 M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 283K