4NBS
The structure of aIF2gamma subunit H20F from archaeon Sulfolobus solfataricus complexed with GDPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-04 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 187.152, 187.152, 187.152 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.788 - 2.305 |
| R-factor | 0.1787 |
| Rwork | 0.177 |
| R-free | 0.20140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3SJY |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.009 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1389)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.790 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.576 | |
| Number of reflections | 48037 | |
| <I/σ(I)> | 2.41 | |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 6.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 301 | 4M sodium formiate, 0.1M sodium cacodylate, 1% MMEPEG 5K, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
