4N9J

Crystal structure of the cryptic polo box domain of human Plk4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	CCD
Collection date	2013-04-09
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97949
Spacegroup name	P 61 2 2
Unit cell lengths	128.063, 128.063, 168.357
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.108 - 2.601
R-factor	0.2053
Rwork	0.202
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.294
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	25386

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	100 mM Bis-Tris (pH 5.5), 2.0 M ammonium sulfate, 1% (v/v) methanol , VAPOR DIFFUSION, SITTING DROP, temperature 295K