4N99
E. coli sliding clamp in complex with 6-chloro-2,3,4,9-tetrahydro-1H-carbazole-7-carboxylic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-08-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.010, 65.130, 73.240 |
| Unit cell angles | 72.95, 85.06, 85.24 |
Refinement procedure
| Resolution | 40.950 - 2.300 |
| R-factor | 0.2647 |
| Rwork | 0.261 |
| R-free | 0.33780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k3s |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.877 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 69.874 | 40.949 | 2.420 |
| High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
| Rmerge | 0.052 | 0.643 | |
| Total number of observations | 3308 | 15628 | |
| Number of reflections | 29744 | ||
| <I/σ(I)> | 8.5 | 11.6 | 1.2 |
| Completeness [%] | 92.9 | 95.8 | 89.9 |
| Redundancy | 3.8 | 3.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
