4N96
E. coli sliding clamp in complex with 6-nitroindazole
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-04 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.95 |
Spacegroup name | P 1 |
Unit cell lengths | 40.880, 64.670, 72.020 |
Unit cell angles | 73.97, 83.55, 84.36 |
Refinement procedure
Resolution | 28.720 - 1.700 |
R-factor | 0.2297 |
Rwork | 0.227 |
R-free | 0.27480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k3s |
RMSD bond length | 0.008 |
RMSD bond angle | 1.324 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.21) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 68.945 | 28.900 | 1.790 |
High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
Rmerge | 0.032 | 0.328 | |
Total number of observations | 8748 | 33284 | |
Number of reflections | 64167 | ||
<I/σ(I)> | 15.2 | 15.4 | 2.2 |
Completeness [%] | 83.0 | 96.2 | 80.4 |
Redundancy | 3.8 | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | 100mM MES, 100-150mM CaCl2, 25-30%(v/v) PEG400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |