4N83
X-ray crystal structure of Streptococcus sanguinis dimanganese(II)-NrdF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-03 |
Detector | MARRESEARCH |
Wavelength(s) | 1.078 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 117.657, 80.224, 166.374 |
Unit cell angles | 90.00, 105.91, 90.00 |
Refinement procedure
Resolution | 29.900 - 2.650 |
R-factor | 0.2377 |
Rwork | 0.235 |
R-free | 0.27780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n37 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.806 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BALBES |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.881 |
High resolution limit [Å] | 2.650 |
Number of reflections | 86753 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | PEG 3000, magnesium formate, pH 7.6, vapor diffusion, hanging drop, temperature 298K |