4N7Y
Crystal structure of 14-3-3zeta in complex with a 8-carbon-linker cyclic peptide derived from ExoS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-23 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.510, 103.520, 114.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.940 - 2.160 |
R-factor | 0.2149 |
Rwork | 0.213 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 2.096 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.940 | 2.180 | |
High resolution limit [Å] | 2.160 | 20.000 | 2.160 |
Rmerge | 0.069 | 0.038 | 0.943 |
Number of reflections | 46765 | 78 | 1271 |
<I/σ(I)> | 20.53 | 37.28 | 2.61 |
Completeness [%] | 100.0 | 89.7 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 277.15 | 0.09 M HEPES sodium, pH 7.5, 1.26 M sodium citrate, 10% v/v glycerol, VAPOR DIFFUSION, temperature 277.15K |