4N7V
Crystal structure of human Plk4 cryptic polo box (CPB) in complex with a Cep152 N-terminal fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-02 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 126.551, 63.942, 79.499 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.263 - 2.758 |
R-factor | 0.2583 |
Rwork | 0.258 |
R-free | 0.28760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g7n |
RMSD bond length | 0.004 |
RMSD bond angle | 0.915 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.068 | 0.303 |
Number of reflections | 15869 | |
<I/σ(I)> | 16.9 | 2.06 |
Completeness [%] | 92.6 | 72.7 |
Redundancy | 3.8 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295.15 | 22% PEG 4000, 3% Iso-propanol, 0.1M Tris-HCl, 0.2M Lithium Sulfate , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |