4N71
X-Ray Crystal Structure of 2-amino-1-hydroxyethylphosphonate-bound PhnZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2013-06-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.761, 65.423, 109.355 |
| Unit cell angles | 90.00, 124.83, 90.00 |
Refinement procedure
| Resolution | 29.095 - 2.984 |
| R-factor | 0.2897 |
| Rwork | 0.278 |
| R-free | 0.33340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.452 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 89.000 |
| High resolution limit [Å] | 2.980 |
| Number of reflections | 16580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 294 | 0.1 M sodium acetate, 0.2 M ammonium sulphate, 25%(w/v)PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
