4N3S
Crystal structure of eukaryotic translation initiation factor eIF5B (399-852) from Saccharomyces cerevisiae, apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.826580 |
Spacegroup name | P 41 |
Unit cell lengths | 118.010, 118.010, 77.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.949 - 1.832 |
R-factor | 0.1689 |
Rwork | 0.168 |
R-free | 0.19410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g7r |
RMSD bond length | 0.008 |
RMSD bond angle | 1.141 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 1.930 | ||
High resolution limit [Å] | 1.830 | 50.000 | 1.830 |
Rmerge | 0.039 | 0.525 | |
Number of reflections | 93360 | 13677 | |
<I/σ(I)> | 21.05 | 3.11 | |
Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 5 % PEG 3350 and 20 mM MgCl2., VAPOR DIFFUSION, SITTING DROP, temperature 277K |