4N2Y

Crystal structure of orotidine 5'-monophosphate decarboxylase from Archaeoglobus fulgidus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	36.563, 116.859, 87.147
Unit cell angles	90.00, 90.12, 90.00

Refinement procedure

Resolution	36.563 - 1.549
R-factor	0.1849
Rwork	0.184
R-free	0.20890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4muz
RMSD bond length	0.007
RMSD bond angle	1.037
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	36.563
High resolution limit [Å]	1.549
Number of reflections	105168
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	20% PG monomethyl ether 2000,0.1M Tris, 0.01M Ni chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K