4N19
Structural basis of conformational transitions in the active site and 80 s loop in the FK506 binding protein FKBP12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.369, 35.859, 41.240 |
| Unit cell angles | 90.00, 96.65, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.200 |
| R-factor | 0.1296 |
| Rwork | 0.126 |
| R-free | 0.18040 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2ppn |
| RMSD bond length | 0.002 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.7.3_928) |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.210 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.060 | 0.338 |
| Number of reflections | 23793 | |
| <I/σ(I)> | 48 | 2.4 |
| Completeness [%] | 80.0 | 6 |
| Redundancy | 5.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
