4N19
Structural basis of conformational transitions in the active site and 80 s loop in the FK506 binding protein FKBP12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-14 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.369, 35.859, 41.240 |
Unit cell angles | 90.00, 96.65, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.200 |
R-factor | 0.1296 |
Rwork | 0.126 |
R-free | 0.18040 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2ppn |
RMSD bond length | 0.002 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.7.3_928) |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.210 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.060 | 0.338 |
Number of reflections | 23793 | |
<I/σ(I)> | 48 | 2.4 |
Completeness [%] | 80.0 | 6 |
Redundancy | 5.7 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |