4N14
Crystal structure of Cdc20 and apcin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97937 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.041, 87.177, 48.043 |
Unit cell angles | 90.00, 112.72, 90.00 |
Refinement procedure
Resolution | 28.580 - 2.100 |
R-factor | 0.1678 |
Rwork | 0.165 |
R-free | 0.21290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.734 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.580 | 50.000 | 1.980 |
High resolution limit [Å] | 2.100 | 5.290 | 1.950 |
Rmerge | 0.082 | 0.047 | 0.550 |
Number of reflections | 16859 | ||
<I/σ(I)> | 13.7 | ||
Completeness [%] | 92.8 | 99.7 | 24.4 |
Redundancy | 3.5 | 3.9 | 1.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM MES, 15% (w/v) PEG 6000, and 5% MPD, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |