4N14
Crystal structure of Cdc20 and apcin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.041, 87.177, 48.043 |
| Unit cell angles | 90.00, 112.72, 90.00 |
Refinement procedure
| Resolution | 28.580 - 2.100 |
| R-factor | 0.1678 |
| Rwork | 0.165 |
| R-free | 0.21290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.734 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.580 | 50.000 | 1.980 |
| High resolution limit [Å] | 2.100 | 5.290 | 1.950 |
| Rmerge | 0.082 | 0.047 | 0.550 |
| Number of reflections | 16859 | ||
| <I/σ(I)> | 13.7 | ||
| Completeness [%] | 92.8 | 99.7 | 24.4 |
| Redundancy | 3.5 | 3.9 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM MES, 15% (w/v) PEG 6000, and 5% MPD, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
