4N0H

Crystal structure of S. cerevisiae mitochondrial GatFAB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-17
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	61.949, 86.297, 191.233
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.576 - 1.952
R-factor	0.1897
Rwork	0.188
R-free	0.22180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SeM-labeled yeast GatFAB
RMSD bond length	0.004
RMSD bond angle	0.892
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.980
High resolution limit [Å]	1.950	5.290	1.950
Rmerge	0.118	0.082	0.389
Number of reflections	74495
<I/σ(I)>	7.8
Completeness [%]	98.7	91.9	98.8
Redundancy	5.3	5.6	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	50mM Sodium malonate, 200mM Mg(NO3)2, 10% PEG3350, pH 8.5, vapor diffusion, sitting drop, temperature 293K