4N0D
Crystal structure of the K345L variant of the Gi alpha1 subunit bound to GTPgammaS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 79.635, 79.635, 104.825 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.807 - 1.550 |
R-factor | 0.1585 |
Rwork | 0.157 |
R-free | 0.18740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 2ZJY was used as the starting model. |
RMSD bond length | 0.018 |
RMSD bond angle | 1.786 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.966 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 55216 | |
Completeness [%] | 99.0 | 92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 2.2 M ammonium sulfite, pH 8.0, 20 mM magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |