4N09
Structure of Trypanosoma brucei brucei adenosine kinase in complex with adenosine and AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.530, 90.220, 98.040 |
| Unit cell angles | 90.00, 103.78, 90.00 |
Refinement procedure
| Resolution | 65.040 - 2.600 |
| R-factor | 0.18802 |
| Rwork | 0.186 |
| R-free | 0.22144 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.531 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0033) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 65.040 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 45507 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1 M Bis Tris propane, pH 8.5, 0.2 M sodium citrate, 24 % (w/v) polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
