4MZI
Crystal structure of a human mutant p53
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 85 |
| Detector technology | PIXEL |
| Collection date | 2013-04-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.93340 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.707, 62.428, 73.701 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.250 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.250 |
| Number of reflections | 8315 |
| Completeness [%] | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 278 | pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
