4MZE

Crystal structure of hPIV3 hemagglutinin-neuraminidase, H552Q/Q559R mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	110
Detector technology	PIXEL
Collection date	2012-04-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 21 21 21
Unit cell lengths	84.029, 96.643, 105.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.924 - 1.800
R-factor	0.1626
Rwork	0.161
R-free	0.19610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.625
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.800	1.800
Rmerge	0.062
Number of reflections	79956
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	295.5	20% PEG1000, 0.1 M phosphate-citrate, pH 4.2, 0.1 M lithium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K