4MYH
Structure of the Glutathione bound mitochondrial ABC transporter, Atm1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 157.686, 157.686, 523.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.993 - 3.380 |
| R-factor | 0.2496 |
| Rwork | 0.247 |
| R-free | 0.28990 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.334 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.993 | 3.439 |
| High resolution limit [Å] | 3.380 | 3.380 |
| Number of reflections | 54949 | |
| Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | 35% (w/v) PEE (pentaerythritol ethoxylate), 100mM MES, pH 6.5, 200mM ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
