4MX3
Crystal Structure of PKA RIalpha Homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-09-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 104.672, 104.672, 218.327 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.880 |
| R-factor | 0.26082 |
| Rwork | 0.260 |
| R-free | 0.28671 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rgs |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.853 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.020 |
| High resolution limit [Å] | 3.880 | 3.880 |
| Rmerge | 0.088 | 0.575 |
| Number of reflections | 11860 | |
| <I/σ(I)> | 35.9 | 6.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 0.125 M sodium acetate (pH 5), 2M sodium formate with the protein at a final concentration of 4 mg/ml grown in a 2 ul drop, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
