4MX2
Crystal Structure of adenylosuccinate lyase from Leishmania donovani
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.015, 194.697, 147.507 |
| Unit cell angles | 90.00, 91.31, 90.00 |
Refinement procedure
| Resolution | 34.980 - 1.900 |
| R-factor | 0.2195 |
| Rwork | 0.218 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.960 |
| Data scaling software | XSCALE |
| Phasing software | BALBES |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 | |
| High resolution limit [Å] | 1.880 | 5.600 | 1.880 |
| Rmerge | 0.125 | 0.079 | 0.622 |
| Number of reflections | 269966 | 10728 | 41123 |
| <I/σ(I)> | 5.88 | 12.14 | 1.69 |
| Completeness [%] | 95.1 | 98.7 | 89.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 25 % PEG 3350, 0.2 M LiSO4, 0.1 M BisTris pH 6.5, AMP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
