4MUM
Crystal structure of mitochondrial 5'(3')-deoxy ribonucleotidase alternative spliced variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-31 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97826 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.372, 73.372, 106.672 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.656 - 1.271 |
R-factor | 0.1321 |
Rwork | 0.131 |
R-free | 0.14920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4l6a |
RMSD bond length | 0.020 |
RMSD bond angle | 2.336 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.656 | 46.656 | 1.350 |
High resolution limit [Å] | 1.271 | 3.800 | 1.271 |
Rmerge | 0.036 | 0.020 | 0.608 |
Number of reflections | 76192 | 3151 | 11389 |
<I/σ(I)> | 26.48 | 83.54 | 2.05 |
Completeness [%] | 98.8 | 99.6 | 92.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 20 mM potassium phosphate monobasic, 8% PEG8000, 10% gycerol, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |