4MTS
Ni- and Zn-bound GloA2 at high resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 50.700, 50.700, 327.640 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.560 - 1.800 |
R-factor | 0.1655 |
Rwork | 0.164 |
R-free | 0.20060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mtr |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.560 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.090 | 0.399 |
Number of reflections | 24649 | |
<I/σ(I)> | 25.5 | 8.1 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 19.5 | 18.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.8 | 293 | 31% PEG3350, 0.1 M Tris, 0.2 M magnesium chloride, 10 mM proline, pH 7.8, VAPOR DIFFUSION, temperature 293K |