4MTH

Crystal structure of mature human RegIIIalpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	30.762, 49.528, 92.152
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.130 - 1.470
R-factor	0.187
Rwork	0.185
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.029
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.470
Number of reflections	24610
<I/σ(I)>	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	22% PEG 8000, 0.1M MES pH 6.0, 0.1M NACL, 10MM NAAC, 5% GLYCEROL, VAPOR DIFFUSION, HANGING DROP, temperature 293K