4MSO

X-ray crystal structure of a serine hydroxymethyl transferase in apo form from Burkholderia cenocepacia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	55.280, 72.580, 182.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.300 - 1.400
R-factor	0.1476
Rwork	0.146
R-free	0.18240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dfo
RMSD bond length	0.009
RMSD bond angle	1.698
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		1.440
High resolution limit [Å]	1.400	6.260	1.400
Rmerge	0.047	0.022	0.552
Number of reflections	144410	1757	10565
<I/σ(I)>	23	63.7	3.5
Completeness [%]	99.7	96.5	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	MCSG1 G8: 0.2 M ammonium sulfate, 0.1 M TRIS pH 8.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K