4MSO
X-ray crystal structure of a serine hydroxymethyl transferase in apo form from Burkholderia cenocepacia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.280, 72.580, 182.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.300 - 1.400 |
R-factor | 0.1476 |
Rwork | 0.146 |
R-free | 0.18240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dfo |
RMSD bond length | 0.009 |
RMSD bond angle | 1.698 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.440 | |
High resolution limit [Å] | 1.400 | 6.260 | 1.400 |
Rmerge | 0.047 | 0.022 | 0.552 |
Number of reflections | 144410 | 1757 | 10565 |
<I/σ(I)> | 23 | 63.7 | 3.5 |
Completeness [%] | 99.7 | 96.5 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | MCSG1 G8: 0.2 M ammonium sulfate, 0.1 M TRIS pH 8.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |