4MSD
Crystal structure of Schizosaccharomyces pombe AMSH-like protein SST2 T319I mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-07-07 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.048, 74.117, 64.865 |
| Unit cell angles | 90.00, 113.41, 90.00 |
Refinement procedure
| Resolution | 33.612 - 1.900 |
| R-factor | 0.1876 |
| Rwork | 0.188 |
| R-free | 0.21580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jxe |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.122 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.565 |
| Number of reflections | 39816 | |
| <I/σ(I)> | 18.5 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 0.03 M citric acid, 0.07 M bis-tris propane, pH 7.6, 20% PEG 3350, benzamidine hydrochloride (additive), VAPOR DIFFUSION, SITTING DROP, temperature 293K |
