4MSD
Crystal structure of Schizosaccharomyces pombe AMSH-like protein SST2 T319I mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-07-07 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.048, 74.117, 64.865 |
Unit cell angles | 90.00, 113.41, 90.00 |
Refinement procedure
Resolution | 33.612 - 1.900 |
R-factor | 0.1876 |
Rwork | 0.188 |
R-free | 0.21580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jxe |
RMSD bond length | 0.007 |
RMSD bond angle | 1.122 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.070 | 0.565 |
Number of reflections | 39816 | |
<I/σ(I)> | 18.5 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 0.03 M citric acid, 0.07 M bis-tris propane, pH 7.6, 20% PEG 3350, benzamidine hydrochloride (additive), VAPOR DIFFUSION, SITTING DROP, temperature 293K |