4MS7
Crystal structure of Schizosaccharomyces pombe sst2 catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-10-07 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.150, 69.391, 61.971 |
| Unit cell angles | 90.00, 104.73, 90.00 |
Refinement procedure
| Resolution | 29.967 - 1.673 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.21050 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.121 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.072 | |
| Number of reflections | 51582 | |
| <I/σ(I)> | 20.8 | |
| Completeness [%] | 96.9 | 90.9 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M Ammonium phosphate dibasic, 20 % w/v polyethylene glycol 3350, 30% w/v 1,6- hexanedio, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 8.0 |
