4MS7
Crystal structure of Schizosaccharomyces pombe sst2 catalytic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-10-07 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.150, 69.391, 61.971 |
Unit cell angles | 90.00, 104.73, 90.00 |
Refinement procedure
Resolution | 29.967 - 1.673 |
R-factor | 0.1788 |
Rwork | 0.177 |
R-free | 0.21050 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.121 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.072 | |
Number of reflections | 51582 | |
<I/σ(I)> | 20.8 | |
Completeness [%] | 96.9 | 90.9 |
Redundancy | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M Ammonium phosphate dibasic, 20 % w/v polyethylene glycol 3350, 30% w/v 1,6- hexanedio, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 8.0 |