4MS6
Human Leukotriene A4 Hydrolase in complex with Pro-Gly-Pro analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.766, 87.483, 98.964 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.900 - 1.720 |
| R-factor | 0.17715 |
| Rwork | 0.175 |
| R-free | 0.21465 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dpr |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.929 |
| Data reduction software | PROCESS |
| Data scaling software | PROCESS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.900 | 1.780 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.181 | 0.016 |
| Number of reflections | 71210 | |
| <I/σ(I)> | 10.09 | 1.83 |
| Completeness [%] | 99.7 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7 | 277 | 50 mM Sodium Acetate, 22% (w/v) PEG 8000, 5 mM YbCl3, pH 7.0, LIQUID DIFFUSION, temperature 277K |
