4MQE
Crystal structure of the extracellular domain of human GABA(B) receptor in the apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.080, 113.080, 73.110 |
Unit cell angles | 90.00, 97.58, 90.00 |
Refinement procedure
Resolution | 23.690 - 2.350 |
R-factor | 0.207 |
Rwork | 0.206 |
R-free | 0.23160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f11 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.160 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 113.076 | 113.076 | 2.480 |
High resolution limit [Å] | 2.350 | 7.430 | 2.350 |
Rmerge | 0.042 | 0.026 | 0.483 |
Total number of observations | 4933 | 26056 | |
Number of reflections | 47242 | ||
<I/σ(I)> | 14.8 | 23.3 | 1.6 |
Completeness [%] | 98.9 | 92.7 | 100 |
Redundancy | 3.7 | 3.4 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 10% PEG3350, 20% glycerol, 0.12 M sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |