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4MQE

Crystal structure of the extracellular domain of human GABA(B) receptor in the apo form

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-C
Synchrotron siteAPS
Beamline24-ID-C
Temperature [K]100
Detector technologyCCD
Collection date2011-04-15
DetectorADSC QUANTUM 315
Wavelength(s)0.97918
Spacegroup nameP 1 21 1
Unit cell lengths71.080, 113.080, 73.110
Unit cell angles90.00, 97.58, 90.00
Refinement procedure
Resolution23.690 - 2.350
R-factor0.207
Rwork0.206
R-free0.23160
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4f11
RMSD bond length0.009
RMSD bond angle1.160
Data reduction softwareXDS
Data scaling softwareSCALA (3.3.16)
Phasing softwarePHASER
Refinement softwareBUSTER-TNT
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]113.076113.0762.480
High resolution limit [Å]2.3507.4302.350
Rmerge0.0420.0260.483
Total number of observations493326056
Number of reflections47242
<I/σ(I)>14.823.31.6
Completeness [%]98.992.7100
Redundancy3.73.43.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP727710% PEG3350, 20% glycerol, 0.12 M sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

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