4MQE

Crystal structure of the extracellular domain of human GABA(B) receptor in the apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	71.080, 113.080, 73.110
Unit cell angles	90.00, 97.58, 90.00

Refinement procedure

Resolution	23.690 - 2.350
R-factor	0.207
Rwork	0.206
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f11
RMSD bond length	0.009
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	113.076	113.076	2.480
High resolution limit [Å]	2.350	7.430	2.350
Rmerge	0.042	0.026	0.483
Total number of observations		4933	26056
Number of reflections	47242
<I/σ(I)>	14.8	23.3	1.6
Completeness [%]	98.9	92.7	100
Redundancy	3.7	3.4	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	10% PEG3350, 20% glycerol, 0.12 M sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K