4MOY

Structure of a second nuclear PP1 Holoenzyme, crystal form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 32 2 1
Unit cell lengths	130.788, 130.788, 47.725
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.980 - 2.195
R-factor	0.1531
Rwork	0.151
R-free	0.18520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.184
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.980	2.240
High resolution limit [Å]	2.195	2.195
Number of reflections	24217
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	277	0.1 M Tris, 1 M LiCl, 18% PEG 6000, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K