4MNG
Structure of the DP10.7 TCR with CD1d-sulfatide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-03-24 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.03321 |
Spacegroup name | P 32 |
Unit cell lengths | 229.333, 229.333, 52.646 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.652 - 3.006 |
R-factor | 0.1794 |
Rwork | 0.178 |
R-free | 0.20680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4MNH and 4MQ7 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.795 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 61100 | |
Completeness [%] | 99.2 | 95.7 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 11% PEG 4000 5% isopropanol 2 mM glutathione reduced/oxidized 50 mM tri-sodium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 292K |