4MN3
Chromodomain antagonists that target the polycomb-group methyllysine reader protein Chromobox homolog 7 (CBX7)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.290, 31.860, 38.390 |
| Unit cell angles | 90.00, 119.02, 90.00 |
Refinement procedure
| Resolution | 29.500 - 1.542 |
| R-factor | 0.17416 |
| Rwork | 0.172 |
| R-free | 0.21265 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.139 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 1.580 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Number of reflections | 8874 | |
| Completeness [%] | 90.7 | 86.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 40% ethylene glycol, 0.1M HEPES pH 7.5, 5% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
