4MM3
Crystal structure of SARS-CoV papain-like protease PLpro in complex with ubiquitin aldehyde
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2009-01-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 47.147, 47.147, 332.658 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.653 - 2.752 |
R-factor | 0.1915 |
Rwork | 0.187 |
R-free | 0.27870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.088 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 11977 | |
Completeness [%] | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M HEPES, pH 7.5, 10% isopropanol, 20% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |