4MKO
Crystal structure of the monomeric, cleaved form of the Pore-Forming Toxin Monalysin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Wavelength(s) | 0.9393 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.844, 117.649, 150.584 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.780 - 1.700 |
R-factor | 0.1804 |
Rwork | 0.179 |
R-free | 0.20460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PBD entry 4MJT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.930 |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.093 | 0.445 |
Number of reflections | 126982 | |
<I/σ(I)> | 12.9 | 3.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.7 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1M sodium acetate, pH 4.6, 0.13M zinc acetate, 0.6-1.1M ammonium acetate, 2-7% PEG800, 1.5mM HgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |