4MKG

Crystal structure of a stable adenylate kinase variant AKv8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-05
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	44.620, 61.320, 87.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.080 - 1.450
R-factor	0.16988
Rwork	0.168
R-free	0.20073
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p3j
RMSD bond length	0.025
RMSD bond angle	2.564
Data reduction software	iMOSFLM
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.620	1.530
High resolution limit [Å]	1.450	1.450
Number of reflections	43604
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	1.4M trisodium citrate pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K