4MJH
Human Hsp27 core domain in complex with C-terminal peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ DW |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-03-27 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.280, 48.960, 57.540 |
Unit cell angles | 90.00, 99.95, 90.00 |
Refinement procedure
Resolution | 37.050 - 2.599 |
R-factor | 0.2359 |
Rwork | 0.234 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 0.995 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.050 | 2.670 | |
High resolution limit [Å] | 2.599 | 11.620 | 2.599 |
Rmerge | 0.072 | 0.047 | 0.494 |
Number of reflections | 5331 | 61 | 341 |
<I/σ(I)> | 17.21 | 30.77 | 3.18 |
Completeness [%] | 99.0 | 89.7 | 89.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris, pH 8.5, 0.2 M magnesium chloride, 30% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |