4MJH

Human Hsp27 core domain in complex with C-terminal peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-03-27
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	31.280, 48.960, 57.540
Unit cell angles	90.00, 99.95, 90.00

Refinement procedure

Resolution	37.050 - 2.599
R-factor	0.2359
Rwork	0.234
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	0.995
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.050		2.670
High resolution limit [Å]	2.599	11.620	2.599
Rmerge	0.072	0.047	0.494
Number of reflections	5331	61	341
<I/σ(I)>	17.21	30.77	3.18
Completeness [%]	99.0	89.7	89.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1 M Tris, pH 8.5, 0.2 M magnesium chloride, 30% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K