4MJ2

Crystal structure of apo-iduronidase in the R3 form

Replaces:  4JXO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-05
Detector	RAYONIX MX300HE
Wavelength(s)	0.9793
Spacegroup name	H 3
Unit cell lengths	259.360, 259.360, 71.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.010 - 2.100
R-factor	0.19215
Rwork	0.191
R-free	0.20637
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.006
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.010	2.154
High resolution limit [Å]	2.100	2.100
Rmerge		0.868
Number of reflections	104643
<I/σ(I)>	12.22	2.33
Completeness [%]	99.5	95.32
Redundancy	10.7	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.01 M HEPES, pH 7.5, 0.26 M sodium potassium tartrate, 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 297K, temperature 298K