4MIW
High-resolution structure of the N-terminal endonuclease domain of the Lassa virus L polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2011-05-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.720, 57.720, 134.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.814 - 1.720 |
| R-factor | 0.1661 |
| Rwork | 0.165 |
| R-free | 0.17830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jsb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.050 | 1.800 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.048 | 0.579 |
| Number of reflections | 22935 | |
| <I/σ(I)> | 23.7 | 3.6 |
| Completeness [%] | 97.9 | 99.5 |
| Redundancy | 9.3 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM Tris-Hcl, pH8-9.5, 250 mM MgSO4 and 10% PEG10000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
