4MID
Crystal Structure of Activin A/BMP2 chimera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-05-12 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 32.615, 32.615, 147.358 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.245 - 2.139 |
R-factor | 0.2213 |
Rwork | 0.219 |
R-free | 0.26430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.767 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | BALBES |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 147.358 | 2.194 |
High resolution limit [Å] | 2.139 | 2.139 |
Rmerge | 0.059 | 0.248 |
Number of reflections | 5395 | |
<I/σ(I)> | 23.2 | 2.8 |
Completeness [%] | 97.2 | 76.7 |
Redundancy | 8.1 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | 0.2 M LiSO4, 0.1 M Tris, 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |