4MID

Crystal Structure of Activin A/BMP2 chimera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-05-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	32.615, 32.615, 147.358
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.245 - 2.139
R-factor	0.2213
Rwork	0.219
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.767
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	BALBES
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	147.358	2.194
High resolution limit [Å]	2.139	2.139
Rmerge	0.059	0.248
Number of reflections	5395
<I/σ(I)>	23.2	2.8
Completeness [%]	97.2	76.7
Redundancy	8.1	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	288	0.2 M LiSO4, 0.1 M Tris, 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K