4MI5
Crystal structure of the EZH2 SET domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Detector technology | CCD |
| Collection date | 2008-04-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.2821 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.134, 57.674, 75.501 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.020 - 2.000 |
| R-factor | 0.19931 |
| Rwork | 0.196 |
| R-free | 0.25671 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.978 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 13157 |
| Completeness [%] | 95.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 100mM MES pH 6.5, 30% PEG MME 5K, 200mM Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
