4MGZ
Selective activation of Epac1 and Epac2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.07225 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 125.228, 147.088, 226.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.850 - 3.000 |
| R-factor | 0.23963 |
| Rwork | 0.238 |
| R-free | 0.26435 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cf6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.041 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 41504 | |
| <I/σ(I)> | 4.6 | |
| Completeness [%] | 98.2 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 0.4M (NH4)2SO4, 1.2M LI2SO4, 0.1M CITRATE, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
