4MEZ
Crystal structure of M68L/M69T double mutant TEM-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-10 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.333, 55.071, 77.681 |
| Unit cell angles | 70.83, 81.22, 71.84 |
Refinement procedure
| Resolution | 73.260 - 2.047 |
| R-factor | 0.19495 |
| Rwork | 0.192 |
| R-free | 0.24780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zg4 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.001 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.260 | 2.090 |
| High resolution limit [Å] | 2.047 | 2.047 |
| Rmerge | 0.152 | 0.392 |
| Number of reflections | 31707 | |
| <I/σ(I)> | 11.8 | 3.5 |
| Completeness [%] | 99.2 | 97.2 |
| Redundancy | 7.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295.15 | 1.5M ammonium sulfate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
