4MEC
Crystal structure of RAT Heme oxygenase-1 in complex with ZN(II)-Protoporphyrin IX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-21 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.2000 |
Spacegroup name | P 1 |
Unit cell lengths | 40.059, 73.167, 148.757 |
Unit cell angles | 86.41, 87.62, 86.25 |
Refinement procedure
Resolution | 38.940 - 3.200 |
R-factor | 0.252 |
Rwork | 0.249 |
R-free | 0.29900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dve |
RMSD bond length | 0.008 |
RMSD bond angle | 0.865 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 27085 | |
<I/σ(I)> | 14.4 | 2.7 |
Completeness [%] | 97.8 | 94 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG 4000, 0.2M Sodium Acetate, 0.1M Tris-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |